Fig. 4
From: CRISPR-SKIP: programmable gene splicing with single base editors
Comparison of CRISPR-SKIP with active SpCas9 for inducing exon skipping. CRISPR-SKIP was utilized to target the splice acceptors of RELA exon 7, PIK3CA exon 5, and JAG1 exon 9. In parallel, sgRNAs targeting the same exons were co-transfected with active SpCas9 to induce exon skipping. Analysis by PCR demonstrates that CRISPR-SKIP induced exon skipping at equal or greater rates than active SpCas9 in each of three exons tested