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Fig. 7 | Genome Biology

Fig. 7

From: HmmUFOtu: An HMM and phylogenetic placement based ultra-fast taxonomic assignment and OTU picking tool for microbiome amplicon sequencing studies

Fig. 7

Benchmarking results from chimeric read detection using the “segment placement comparison” algorithm from HmmUFOtu. a Receiver operating characteristic (ROC) curves for detecting simulated chimeric reads from random in silico cross-over events using GreenGenes 97% OTU reference sequences; 10,000 chimeric or non-chimeric simulated reads were used in the respective range for each p-distance subset. ROC curves are calculated by varying the min LOD cut-off from 0 to 10 with a step of 1, then 20 to 100 with a step of 10. b Estimated proportion of chimeric reads in all of the benchmarked real datasets (mock and HMP) by enabling HmmUFOtu’s chimera detection and setting the LOD cut-off at 50. c Differences in “observed species” alpha diversity of the mock community V1 V3 dataset. Paired: original results using raw paired reads; Nonchimera: chimera-filtered results using the same paired reads

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