From: Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
Species | Target site | sgRNA form | Number of mutant lines/plantsa | Number of transgenic rice lines or bombarded embryos of wheat | A•T to G•C frequency (%)b | Genotype of mutations | Heterozygous/homozygous |
---|---|---|---|---|---|---|---|
Rice | OsACC-T1 | sgRNA | 9 | 130 | 6.9 | T4 > C4 (2); T4T7 > C4C7 (7) | 9/0 |
esgRNA | 33 | 160 | 20.6 | T4 > C4 (10); T7 > C7 (2); T4T7 > C4C7 (21) | 32/1 | ||
OsALS-T1 | sgRNA | 16 | 184 | 8.7 | A5 > G5 (16) | 16/0 | |
esgRNA | 42 | 196 | 21.4 | A4 > G4 (1); A5 > G5 (41) | 42/0 | ||
OsCDC48-T3 | sgRNA | 19 | 210 | 9.0 | A5 > G5 (19) | 19/0 | |
esgRNA | 60 | 180 | 33.3 | A5 > G5 (60) | 60/0 | ||
OsDEP1-T1 | sgRNA | 101 | 217 | 46.5 | A4 > G4 (2); A6 > G6 (90); A4A6 > G4G6 (9) | 88/13 | |
esgRNA | 83 | 211 | 39.3 | A4 > G4 (4); A6 > G6 (73); A4A6 > G4G6 (6) | 77/6 | ||
OsDEP1-T2 | sgRNA | 5 | 154 | 3.2 | A6 > G6 (5) | 5/0 | |
esgRNA | 34 | 215 | 15.8 | A5 > G5 (1); A6 > G6 (32); A3A6 > G3G6 (1) | 33/1 | ||
OsNRT1.1B-T1 | sgRNA | 116 | 303 | 38.3 | A6 > G6 (8); A8 > G8 (30); A4A8 > G4G8 (3); A6A8 > G6G8 (75) | 111/5 | |
esgRNA | 149 | 252 | 59.1 | A6 > G6 (6); A8 > G8 (46); A4A8 > G4G8 (2); A6A8 > G6G8 (95) | 136/13 | ||
Wheat | TaDEP1 | esgRNA | 5 | 460 | 1.1 | A8 > G8 (4, AaBBDD; 1, AABbDD) | 5/0 |
TaGW2 | esgRNA | 2 | 480 | 0.4 | A5 > G5 (2, AABbDD) | 2/0 |