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Table 1 Mutation frequencies induced by PABE-7 in the T0 rice and wheat plants

From: Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

Species

Target site

sgRNA form

Number of mutant lines/plantsa

Number of transgenic rice lines or bombarded embryos of wheat

A•T to G•C frequency (%)b

Genotype of mutations

Heterozygous/homozygous

Rice

OsACC-T1

sgRNA

9

130

6.9

T4 > C4 (2); T4T7 > C4C7 (7)

9/0

esgRNA

33

160

20.6

T4 > C4 (10); T7 > C7 (2); T4T7 > C4C7 (21)

32/1

OsALS-T1

sgRNA

16

184

8.7

A5 > G5 (16)

16/0

esgRNA

42

196

21.4

A4 > G4 (1); A5 > G5 (41)

42/0

OsCDC48-T3

sgRNA

19

210

9.0

A5 > G5 (19)

19/0

esgRNA

60

180

33.3

A5 > G5 (60)

60/0

OsDEP1-T1

sgRNA

101

217

46.5

A4 > G4 (2); A6 > G6 (90); A4A6 > G4G6 (9)

88/13

esgRNA

83

211

39.3

A4 > G4 (4); A6 > G6 (73); A4A6 > G4G6 (6)

77/6

OsDEP1-T2

sgRNA

5

154

3.2

A6 > G6 (5)

5/0

esgRNA

34

215

15.8

A5 > G5 (1); A6 > G6 (32);

A3A6 > G3G6 (1)

33/1

OsNRT1.1B-T1

sgRNA

116

303

38.3

A6 > G6 (8); A8 > G8 (30); A4A8 > G4G8 (3); A6A8 > G6G8 (75)

111/5

esgRNA

149

252

59.1

A6 > G6 (6); A8 > G8 (46); A4A8 > G4G8 (2); A6A8 > G6G8 (95)

136/13

Wheat

TaDEP1

esgRNA

5

460

1.1

A8 > G8 (4, AaBBDD;

1, AABbDD)

5/0

TaGW2

esgRNA

2

480

0.4

A5 > G5 (2, AABbDD)

2/0

  1. aThe number of mutant lines for rice and the number of mutant plants for wheat. b Based on the number of T0 lines (rice) or plants (wheat) carrying the observed mutations over the total number of T0 transgenic rice lines analyzed or bombarded immature embryos of wheat