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Fig. 2 | Genome Biology

Fig. 2

From: Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

Fig. 2

Analysis of PABE-7 activity on endogenous genes using different sgRNA expression constructs. a Frequencies of targeted single A to G conversions in the 13 target sites of rice and wheat genes. The native forms of sgRNA, esgRNA, and tRNA-sgRNA were used. b Summary of the A to G conversion activities of PABE-7 in a. c Frequencies of indels in the 13 target sites of rice and wheat genes. d The effect of spacer length of sgRNA on editing efficiency. A to G editing frequencies induced by the PABE-7 and esgRNAs of different length varying from 14 to 20 nucleotides were determined at protospacer positions 2–9. In a, c, and d, an untreated protoplast sample was used as control and each frequency (mean ± standard error of the mean) was calculated using the data from three independent biological replicates (n = 3). e OsACC-T1 with C2186R substitution confers resistance to herbicide. Sequence alignment comparing WT OsACC-T1 with that in the T0–13 mutant. Phenotypes of T0–13 with C2186R substitution in the regeneration medium supplemented with 0.086 ppm haloxyfop-R-methyl. Scale bars, 1 cm

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