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Fig. 4 | Genome Biology

Fig. 4

From: Hepatic steatosis risk is partly driven by increased de novo lipogenesis following carbohydrate consumption

Fig. 4

a Hepatic TAG changes associated with high fat or regular chow feeding in ob/ob mice. Using partial least squares discriminant analysis (PLS-DA) to assess the changes in the TAGs within the murine liver demonstrates a shift toward a higher proportion of TAGs with fewer double bonds and carbon atoms when fed regular chow than when fed a high fat diet. Each point’s area reflects the Variable Importance Parameter score for the PLS-DA model. b A simple linear model of the summated TAG Fenland cluster 3 signal against calculated de novo synthesised palmitate. Plotting the total normalised TAG cluster 3 signal against the total palmitate calculated to be synthesised from DNL using deuterium incorporation revealed a significant positive correlation between the two measurements (R2 = 0.82, p < 1*10−5). c Plasma TAG changes before and after a high carbohydrate meal. Using PLS-DA to assess the changes in the TAGs within the plasma lipidome demonstrates a shift toward a higher proportion of TAGs with fewer double bonds and carbon atoms 3.5 h after the high carbohydrate meal compared to before the meal in the fasting state. Each point’s area reflects the Variable Importance Parameter score for the PLS-DA model. d A simple linear model of the summated TAG Fenland cluster 3 signal against calculated de novo synthesised palmitate. Plotting the total normalised TAG cluster 3 signal against the proportion of palmitate calculated to be synthesised from DNL using deuterium incorporation revealed a significant positive correlation between the two measurements (R2 = 0.89, p < 5*10−6)

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