Fig. 1From: Single-cell profiling of human gliomas reveals macrophage ontogeny as a basis for regional differences in macrophage activation in the tumor microenvironmentScRNA-seq of neoplastic and immune cells from human primary gliomas. a Both whole-tumor and CD11b-purified single-cell suspensions, derived from glioma biopsies, were subjected to scRNA-seq (top) allowing for quantification of markers in single cells from both populations (bottom). b t-distributed stochastic neighbor embedding plot of cells from whole-tumor and CD11b-purified scRNA-seq, colored by the presence of somatic mutations that are clonal in exome sequencing (top) or by the expression of canonical marker genes (bottom), measured in counts per million (CPM). c Hierarchical clustering of cells (columns), grouped by their expression of canonical marker genes (rows)Back to article page