Fig. 7

The relationship between PABP occupancy and transcript stability. a The relationship between mean poly(A)-tail length and mRNA half-life. Results are plotted for each gene that exceeded the expression cutoffs for quantification. b The relationship between PABP occupancy and mRNA half-life; otherwise as in a. c The top 21 gene ontology (GO) terms enriched in genes more unstable than predicted by their PABP occupancies, plotting for each term the log-transformed q value of its enrichment. Dashed line indicates a q-value of 0.001. d eIF4E, eIF4G, and PABP occupancies for mRNAs of mitochondrial ribosomal protein genes (blue), cytoplasmic ribosomal protein genes (green), and all other genes that exceeded the expression cutoffs for quantification (black) (line, median; box, quartiles; whiskers, 1.5 interquartile range). Significance was evaluated using the two-tailed K–S test. e Mean poly(A)-tail lengths of mRNAs of mitochondrial ribosomal protein genes, cytoplasmic ribosomal protein genes, and other genes; otherwise as in d. f mRNA stabilities of mRNAs of mitochondrial ribosomal protein genes, cytoplasmic ribosomal protein genes, and other genes; otherwise as in d. g The top 27 GO terms enriched in genes more stable than predicted by their PABP occupancies. Otherwise, this panel is as in c