Fig. 5

The weak relationship between mean poly(A) tail length and PABP occupancy in human cells. a Comparison of eIF4E and eIF4G occupancies for mRNAs of each gene that exceeded the expression cutoffs for quantification. b Comparison of eIF4E and PABP occupancies; otherwise as in a. c Comparison of eIF4G and PABP occupancies; otherwise as in a. d Relationship between PABP occupancy and mean poly(A)-tail length for mRNAs of each gene that exceeded the expression cutoffs for quantification. e Mean poly(A)-tail lengths of mRNAs of ribosomal protein genes (red) and of mRNAs of other genes (black). Significance was evaluated using the two-tailed K–S test. f eIF4E, eIF4G, and PABP occupancies for mRNAs of ribosomal protein genes (red) and those of other genes exceeding the expression cutoff (black). Significance was evaluated using the two-tailed K–S test. g Phasing analysis of poly(A)-tail lengths of total mRNA. For each of 100 randomly selected genes, each pairwise difference of individual tail lengths was calculated, and the normalized frequency for each possible difference was plotted (grey points) together with the average frequency (red points). h A zoomed-in version of g, focusing on differences between 0 and 60 nucleotides. i Phasing analysis of poly(A)-tail lengths of PABP-bound mRNA; otherwise, as in g. j A zoomed-in version of i, focusing on differences between 0 and 60 nucleotides