Fig. 1From: Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavageSpCas9 variants employed in these studies. Schematics depicting the main features of the wild type and the five mutant variants of SpCas9 used: each protein sequence is flanked by a nuclear localization signal (NLS) at both ends and is preceded by a 3xFLAG tag. HeF-variants containing combinations of mutations from both eSpCas9 and SpCas9-HF1Back to article page