Fig. 3

scUMCs are enriched in chromatin interactions. a, b scUMC (a) and conserved UMR (b) enrichment for 161 transcription factors (TF); the x-axes represent the log2 ratio of observed vs. expected number of features overlapping each TF; the y-axes represent the percentage of features bound by each TF. For a more accurate comparison with the genome-wide distribution of scUMCs, distal conserved UMRs (greater than ± 3 kb from TSS) are plotted (b). c Percentage of distal epigenetic features (red, scUMC; blue, UMR) in B cells overlapping chromatin-looping factors or anchors of chromatin interactions. Chromatin-looping factor sites represent distal regions (greater than ± 3 kb from TSS) co-occupied by Rad21, Znf143, and CTCF ChIP-seq peaks in GM12878 B cells (ENCODE). Anchors represent chromatin-looping interactions of Rad21 measured by ChIA-PET in GM12878 B cells [14]. d Average methylation level in B cells of chromatin-looping factor sites containing either scUMCs or UMRs. e Average occupancy of chromatin looping factors (Rad21, Znf143, and CTCF) centered on either scUMCs or UMRs in B cells. f Interaction intensity of anchor regions overlapping either scUMCs or UMRs. The loop interaction score is as published in Heidari et al. [14]