Fig. 6

Translation repressive sequence elements are preserved in the 5′ end region of the JPX lncRNA. a Scheme and sequences of the JPX regions inserted into the pGL3 basic plasmid. On the bottom, the 5′ end of the insert with the mutated codons highlighted. The TISU element is in bold and the ORF is underlined. b Firefly luciferase levels (normalized by co-transfected Renilla luciferase and by the pGL3-Control levels). c Changes in firefly luciferase relative levels following the indicated mutations, normalized to co-transfected Renilla luciferase and to the pGL3-JPX construct; d Quantification of relative JPX transcript levels in HEK293 mutant cells compared to wild-type (WT) cells. β-Actin served as an internal control; bars show average ± SEM, n = 3