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Fig. 3 | Genome Biology

Fig. 3

From: Histone H3 lysine 36 methylation affects temperature-induced alternative splicing and flowering in plants

Fig. 3

H3K36me3 contributes to regulation of temperature-induced splicing. a The graph shows distribution of differences in PSI between 16 °C and 25 °C (ΔPSI) of significant DiS events identified in WT and corresponding ΔPSI distributions in sdg8-2 and sdg26-1 (Additional file 2: Figure S2C). b Graph shows plot of empiric cumulative distribution function (ecdf) for absolute ΔPSI of WT, sdg8-2, and sdg26-1 for significant DiS events in the comparison of WT 16 °C and 25 °C. Distribution of ΔPSI are significantly different between WT and sdg8-2 or sdg26-1 according to the Kolmogorov–Smirnov test c Partial gene model of two examples depicting the DiS event and corresponding plot area showing RNA-seq results. Reads within the AS regions are shown in black, other reads are in gray. At3g08840 is only significantly DiS in the WT while At3g55080 is significantly DiS in WT as well as in sdg26-1. d Gene ontology enrichment for genes of which the splicing is affected by ambient temperature conditions in WT, sdg8-2, and sdg26-1. The histogram includes all overrepresented categories with at least five genes and p value < 0.05 for at least one genotype

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