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Fig. 2 | Genome Biology

Fig. 2

From: The developmental regulator PKL is required to maintain correct DNA methylation patterns at RNA-directed DNA methylation loci

Fig. 2

Map-based cloning of the rdm18 mutations. a A diagram showing the mapped genomic region of rdm18-1. Genetic markers and their positioning on the chromosome are indicated on top of the arrow. b A diagram showing the gene structure of PKL and mutations identified in the rdm18 mutants. The dashed line indicates a whole gene deletion identified in the rdm18-1 mutant. c Bioluminescence phenotype of the T2 plants from the PKL-FLAG transformation of ros1-1 (–/–) rdm18-2 (+/–) plants. d The pkl-1 mutation released silencing at the RD29A promoter in the ros1-1 background. The F3 seedlings with indicated genotypes from pkl-1 (Col) x ros1-1 (C24) crosses were subjected to luminescence imaging after cold treatment for three days. e The transcript level of the ROS1 gene decreases in the pkl-1 mutant. Relative transcript level measured by real-time PCR is shown and the level in WT (Col-0) is arbitrarily set to 1. Error bars represent standard deviations calculated from three biological replicates

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