Fig. 1

RDM18 promotes transcriptional gene silencing at RdDM loci. a Bioluminescence phenotype of two-week-old ros1 rdm18 seedlings. b The ros1 rdm18 mutants exhibit multiple developmental defects. Shown in the figure includes dwarfism, short and curled siliques, and small leaves (eight-week-old plants). c Bioluminescence phenotype of F1 plants generated from crosses between ros1-1 rdm18-1 and ros1-1 rdm18-2. Cauline leaves from six-week-old plants were used for the analyses. d Transcript levels of the pRD29A-LUC transgene and endogenous RD29A gene examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Relative transcript levels were shown with non-treated C24 set to one. Error bars indicate standard deviations calculated from three biological replicates. e Transcript levels of typical RdDM loci measured by RT-PCR. Two independent RT-PCR experiments were performed and the results are shown in two separate panels. LUC: transgene pRD29A-LUC, RD29A endo: endogenous RD29A gene. Ethidium bromide stained agarose gel (total RNA) and no reverse transcriptase PCR (no RT) serve as the loading control and the negative control respectively