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Fig. 3 | Genome Biology

Fig. 3

From: H2A monoubiquitination in Arabidopsis thaliana is generally independent of LHP1 and PRC2 activity

Fig. 3

H3K27me3 marks and LHP1 are dispensable for H2AK121ub marking in A. thaliana. ac Metagene plot showing H2AK121ub coverage at a all marked genes, b H2AK121ub/H2AK121ub/H3K27me3-marked genes,and c only-H2AK121ub-marked genes in WT and clf28/swn7 mutants. df Metagene plot showing H2AK121ub coverage at d all marked genes, e H2AK121ub/H2AK121ub/H3K27me3-marked genes, and f only-H2AK121ub-marked genes in WT and lhp1 mutants. g Western blot quantification of H2AK121ub levels normalized to H3 levels in clf28/swn7. Error bars represent standard deviation among at least three biological replicates (see also Additional file 1: Figure S9). h Levels of H2AK121ub marks at H2AK121ub/H3K27me3-marked genes in clf28/swn7 and lhp1 mutants compared to WT. Percentage of genes with different levels of the marks is indicated by the shade of red (80–120% is considered WT levels)

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