Fig. 4

Differential sensitivity of Xi genes to reprograming-mediated reactivation is validated by SNP-specific Taqman probes and extended to four distinct hF clones. a, b Histogram plots show Xi-specific allelic expression of human X-linked genes in hFxmESC at 0, 3, 4 and 6 days after fusion. Genes that accordingly to RNA-seq are reactivated (green) or remain inactive (red) upon reprograming or that already have significant Xi expression in clones 12 or 34 ahead of reprogramming (purple) are shown and their locus positions are highlighted along the chromosome ideogram as vertical lines. Allelic expression was measured by Taqman SNP-specific probes and data represented as a percentage of the Normalised Relative Fluorescence Units (NRFU) for the Xi probe vs. the total (Xi + Xa + background in no template control). Data represent the average of at least five independent experiments for each clone ± SEM; day 3 samples were collected only in two independent experiments. Asterisks (*) mark significant differences vs. day 0 (p <0.05, two-sided t-test)