Fig. 2

Cloning of the barley Eceriferum-q and the wheat Pm2 genes by MutChromSeq. a Wax covered leaf sheath of wild-type barley cultivar Foma and six eceriferum (not bearing wax) mutants. Line 334 was deemed to be a cultivar contaminant following sequence analysis. b Powdery mildew infected wheat leaves of line CI12632/8*Cc (Pm2), six EMS-derived susceptible mutants (pm2) and the susceptible control cultivar Chancellor. c, d The Eceriferum (c) and Pm2 (d) loci showing intron–exon boundaries, protein domains and 5′ and 3′ untranslated regions (UTR). Mutations identified by MutChromSeq are indicated by red vertical lines, while mutations identified by Sanger sequencing of additional mutants are indicated by black vertical lines. A number above the line indicates identical mutations occurring in independent lines. Non-sense mutations are indicated by asterisks. Two mutations identified in the pm2 mutant 80_355 are joined by an arched line. CC, coiled-coil; NB-ARC, nucleotide-binding adaptor shared by APAF-1, R proteins, and CED-4; LRR, leucine-rich repeat