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Fig. 4 | Genome Biology

Fig. 4

From: An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease

Fig. 4

The effect of the length of the gDNA on the DNA cleavage by SGN. Schematic (top) shows the gDNAs next to the ssDNA targets. The red arrows indicate the possible cleavage sites according to the cleaved products sizes. The blue arrows indicate the theoretical cleavage sites. The denatured-PAGE results (bottom) show the clearly cleaved products by SGN. (a) S-6 ssDNA target with gDNA-6-10 nt, gDNA-6-15 nt, gDNA-6-20 nt, gDNA-6-25 nt, gDNA-6-30 nt, gDNA-6-35 nt, gDNA-6-40 nt, gDNA-6-45 nt, gDNA-6-50 nt, gDNA-6-55 nt, and gDNA-6-60 nt; (b) S-7 ssDNA with gDNA-7-10 nt, gDNA-7-15 nt, gDNA-7-20 nt, gDNA-7-25 nt, gDNA-7-30 nt, gDNA-7-35 nt, gDNA-7-40 nt, gDNA-7-45 nt, gDNA-7-50 nt, gDNA-7-55 nt, and gDNA-7-60 nt

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Genome Biology

ISSN: 1474-760X

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