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Fig. 2 | Genome Biology

Fig. 2

From: An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease

Fig. 2

SGN cleaved ssDNA in vitro. Denatured-PAGE showed the DNA products produced by SGN cleavage. ac Different target ssDNAs (S-1, S-2, and S-3) were reacted with different gDNAs (gDNA-1, gDNA-2, and gDNA-3). Lane 1: S plus SGN; Lane 2: S plus gDNA; Lane 3: S plus gDNA and Fok I; Lane 4: S plus gDNA and FEN-1; Lane 5: S plus gDNA and SGN. NC: no-enzyme control. Lane M: DNA standard. The red arrows (on the top of each panel) indicate the possible cleavage sites according to the sizes of the cleaved products

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