Fig. 6From: Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stabilityElevated H4K20me3 levels reinforce a stable proliferative arrest in senescent cells. a Western blot of indicated proteins from whole cell extracts of IMR90 cells 12Â days after infection with vector control (CON), Myc-tagged SUV420H1 (H1), Myc-tagged SUV420H2 (H2) or H-RASG12V (OIS). b Western blot of histone H4 and indicated H4K20 modifications from whole cell extracts described in a, normalized for total histone H4 content. c Immunofluorescent images of Myc-tagged SUV420H1 or SUV420H2 and H4K20me3 in CON, H1, and H2 IMR90 cells from a. d Growth curves expressed as cumulative population doublings for CON, H1, and H2 IMR90 cells measured for 38Â days after infection. e Western blot of indicated proteins from whole cell extracts of CON, H1, and H2 IMR90 cells from d 3 and 38Â days after infection. f Western blot of indicated proteins from whole cell extracts of CON and H2 IMR90 cells collected 5, 10, or 15Â days after infection with either vector control (CON) or H-RASG12V (OIS); (s) and (l) denote short and long autoradiographic exposures, respectivelyBack to article page