Fig. 5

Metagenomic clustering of ocean and human metagenomes using Mash. a Comparison of Global Ocean Survey (GOS) clustering using Mash (top left) and COMMET (top right) using raw Sanger sequencing data. Heat maps illustrate the pairwise similarity between samples, scaled between 0 (white) and 100 (red) for comparison to COMMET. Sample groups are identified and colored using the same key as in Rusch et al. [35]. The Mash clustering identifies two large clusters of temperate and tropical water samples as well as subgroupings consistent with the original GOS study. b Human metagenomic samples combined from the HMP and MetaHIT projects clustered by Mash from 888 sequencing runs (bottom left) and 879 assemblies (bottom right). For both sequencing reads and assemblies, Mash successfully clusters samples by body site and appropriately clusters MetaHIT and HMP stool samples together, even though these samples are from different projects with different protocols