Fig. 4
From: Exploiting single-cell expression to characterize co-expression replicability
Functional connectivity is dependent on expression level in UMI networks. a A UMI-based network is plotted as a function of gene expression level, with co-expression values for highly expressed genes in the bottom left corner and lowly expressed genes in the top right corner. A characteristic sunset pattern is observed in A, with the highest co-expression values occurring between highly expressed genes. b AUROC for synaptic set performance in n-fold cross-validation is plotted against median expression level for each held-out gene (plot shows the results from one network). A strong positive relationship was observed between expression level and performance (mean rs = 0.72 +/– 0.038). Inset: Box-and-whisker plot of synaptic set performance in networks made from all genes compared to networks made from high expressing genes only (median > =16 in each batch). A significant reduction in performance was observed in filtered networks (mean AUROC in all gene networks = 0.73 +/– 0.004, mean AUROC in high expressing networks = 0.49 +/– 0.03, p <0.0002 Wilcoxon rank sum test, n = 8)