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Fig. 5 | Genome Biology

Fig. 5

From: A transcriptome-based global map of signaling pathways in the ovarian cancer microenvironment associated with clinical outcome

Fig. 5

Genes coding for components of lipid signaling expressed in ovarian cancer cells and/or TAMs (RNA-Seq). a, b Genes coding for enzymes involved in the generation lipid mediators and their receptors. Values represent the ratio of expression in tumor cells versus TAMs (median and 95 % CI). Color code as in Fig. 2. Detailed results are summarized in Additional file 3: Datasets S6-S9. c Validation of RNA-Seq data by RT-qPCR of tumor cell samples (each dot represents a different sample). d RT-qPCR analysis of matched tumor cell and TAM samples from the same patients (each dot represents a matched pair). Data are represented as the ratio of expression in tumor cells and TAMs. The dotted line indicates a ratio of 1. Data in (c) and (d) were statistically analyzed by Student’s t-test (two-sided, unequal variance). e Concentrations of lipid mediators in the ascites fluid from ovarian cancer patients determined by LC-MS/MS. Each dot represents a different patient, horizontal lines indicate the median. f Concentrations of autotaxin (ENPP2) and PLA2 isoforms in ascites quantified by ELISA

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