Fig. 3
From: Pooling across cells to normalize single-cell RNA sequencing data with many zero counts
Schematic of the deconvolution method. All cells in the data set are averaged to make a reference pseudo-cell. Expression values for cells in pool A are summed together and normalized against the reference to yield a pool-based size factor θ A . This is equal to the sum of the cell-based factors θ j for cells j=1–4 and can be used to formulate a linear equation. (For simplicity, the t j term is assumed to be unity here.) Repeating this for multiple pools (e.g., pool B) leads to the construction of a linear system that can be solved to estimate θ j for each cell j