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Fig. 3 | Genome Biology

Fig. 3

From: Single-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways

Fig. 3

MEP contain three distinct subpopulations segregated by differential expression of megakaryocyte and erythroid-associated genes. a PCA of 681 cells showing distribution of unselected MEP cells (n = 489; red) and CD71 + 41+ selected MEP (n = 192; blue) for PC 1 (8.95 % variance) and PC2 (5.94 % variance). CD71 + 41+ MEP are distinct from Populations 1 and 2. b The three subpopulations that emerged from the PCA (Fig. 2a) were defined as Populations 1 (green), 2 (purple), and 3 (orange) on the basis of PC1 and PC2 values. c The 18 most highly weighted genes in PC1 and 2 show that the distinction of the populations is driven by differential expression of key megakaryocyte (orange font) and erythroid-associated (purple font) genes. Blue font indicates genes associated with more primitive cellular phenotype (CD44 and KIT). Black indicates an MEP gene (DHRS3) and yellow (GATA1, CD36) genes expressed in both megakaryocytic and erythroid cells. d Heatmap of Ct values shows differential gene expression of 20 selected genes between the three populations identified on the PCA. (Green, Population 1; purple, Population 2; orange, Population 3)

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