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Fig. 3 | Genome Biology

Fig. 3

From: CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

Fig. 3

Cell-cycle associated differences in the transcriptome. a Experimental system. GFP+ cells are in the S, G2, and M phases of the cell cycle and a growing culture is composed of roughly equal numbers of GFP+ and GFP– cells. Trypsinized cells are either loaded directly onto the Fluidigm C1 with GFP signal observed in the chip, or sorted according to the GFP signal and manually frozen. b Coefficient of variation on the C1. Each dot indicates a gene’s squared coefficient of variation. c PCA of the genes with high coefficient of variation (labeled blue in (b)). d Gene Ontology (GO) terms of differentially expressed genes – PC3 > 0 vs. PC3 < 0 in (c)

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