Fig. 8From: In silico lineage tracing through single cell transcriptomics identifies a neural stem cell population in planariansModel of planarian stem cell hierarchies. a PCA for predicted pluripotent and lineage-committed groups from the current study and [40]. Colored arrows indicate separate lineages. b–e PCA plots with the following lineages removed: b neural lineage; c novel X2/Group 4 lineage; d epithelial lineage; e gut lineage. f Summary model of planarian lineages. Based on the scRNAseq and Waterfall/pseudotime analyses, we hypothesize that cNeoblasts are represented in our Group 2 cluster, which give rise to pluripotent Group 1/σNeoblasts. In turn, σNeoblasts give rise to ζ, γ, ν, and Group 4 neoblasts, represented in the middle tier. We hypothesize that these neoblast subclasses give rise to tissue-specific lineages on the third tier, such as epithelium for ζNeoblasts, gut for γNeoblasts, and neurons for νNeoblasts. Red question marks denote either unknown existence or unknown ability to self-renewBack to article page