Fig. 5

Transactivation domain is required for chromatin reprogramming. a Read density heatmaps showing the signal enrichment of Ty1 ChIP-seq, ATAC-seq, H3K4me1, and K27ac ChIP-seq in control, wild-type, and mutant GATA3 expressing cells. The same classification was utilized, but only the sites where both wild-type and mutant were localized are represented. The number of peaks in each category is reported. Each row indicates a 10 kb window centered on a GATA3 binding site. The scale of read density after normalization is indicated at the bottom right. Metagene profiles of normalized ATAC-seq (b), H3K4me1 ChIP-seq (c), and H3K27ac ChIP-seq (d) in G1 are shown for the comparison of the average signal levels in control, wild-type GATA3, and TA1del mutant expressing cells. Metagene profiles in the other groups are shown in Additional file 1: Figure S5. Metagene profiles of normalized MNase-seq signal density in G1 (e), G2 (f), and G3 (g) are shown, respectively. Top panels indicate the comparison between control and wild-type GATA3 expressing cells. Bottom panels indicate the comparison between control and TA1 del mutant expressing cells. Only overlapped peaks between wild-type and mutant GATA3 were used for the metagene analyses (b-g)