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Fig. 4 | Genome Biology

Fig. 4

From: GATA3-dependent cellular reprogramming requires activation-domain dependent recruitment of a chromatin remodeler

Fig. 4

N-terminal truncation mutant binds but fails to open chromatin. a Schematic representation of wild-type GATA3 and N-terminal domain lacking (TA1del) mutant. b Genomic distribution of TA1del mutant. ChIP-seq with antibody against GATA3 or Ty1 is shown in purple or dark blue, respectively. ChIP-seq signals in wild-type GATA3 expressing cells were represented in the top and middle panels, while ChIP-seq signals in TA1del mutant expressing cells were shown in the bottom panel. c Venn diagram showing the peak overlap between wild-type GATA3 and TA1del mutant. d The top motif identified by de novo motif analysis is represented with P values. ChIP-seq peaks of TA1del mutant were analyzed by HOMER software. e Representative genomic loci showing that TA1del mutant expressing cells do not exhibit increased levels of ATAC-seq, H3K4me1 and K27ac signals as compared with wild-type GATA3 expressing cells. f Metagene profiles of normalized ATAC-seq signals showing the average tag density in TSS-proximal or -distal (>1 kb) peak flanking regions. The x-axis indicates the distance from the midpoint of TA1del mutant ChIP-seq peaks

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