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Fig. 2 | Genome Biology

Fig. 2

From: GATA3-dependent cellular reprogramming requires activation-domain dependent recruitment of a chromatin remodeler

Fig. 2

Identification and characterization of ‘Pioneer’ sites bound by GATA3. a Read density heatmaps showing the signal enrichment of GATA3 ChIP-seq, ATAC-seq, H3K4me1, and H3K27ac ChIP-seq. Classification from G1 to G4 was carried out based on ATAC-seq signal changes between control and GATA3 expressing cells. The number of peaks in each category is reported below the group label. Each row indicates a 10 kb window centered on a GATA3 binding site. The scale of read density after normalization (see Additional file 1: Table S6) is indicated at the bottom right. Metagene profiles of normalized ATAC-seq (b), H3K4me1 ChIP-seq (c), and H3K27ac ChIP-seq (d) in G1 are shown for the comparison of the average signal levels in control and GATA3 expressing cells. Metagene profiles in the other groups are shown in Additional file 1: Figure S2. e The signal enrichment of GATA3 ChIP-seq in each group is shown as a box-and-whisker plot. The top and bottom whiskers show 5th and 95th percentile, respectively. The horizontal line and cross mark the median and mean. *P <0.0001, Mann-Whitney test. Metagene plots of normalized tag density of GATA3 ChIP-seq in G1 (f) and G3 (g) performed with or without thermal treatment at 37 °C. The metagene plot in G2 and the quantitative analysis are represented in Additional file 1: Figure S2

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