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Fig. 4 | Genome Biology

Fig. 4

From: The aspirin-induced long non-coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formation

Fig. 4

OLA1P2 interacted directly with phosphorylated STAT3 (Tyr705). a RIP analysis determined the recovery of OLA1P2 in COLO205 cells using STAT3 or phosphorylated STAT3 antibodies. b RNA pull-down analysis determined the phosphorylated STAT3 protein-lncRNA OLA1P2 interaction in COLO205 cells using 5′ biotin-linked RNAs. c After cells were transfected with the STAT3 expression vector (wt/Y705R) for 48 h, RNA pull-down analysis determined the STAT3 protein-OLA1P2 interaction in COLO205 cells using 5′ biotin-linked RNAs. d RNA FISH technology and immunofluorescence analysis determined the co-localization of OLA1P2 and phosphorylated STAT3 (Tyr705) protein in COLO205 cells. e RIP analysis to determine the recovery of a portion of OLA1P2 in COLO205 cells transfected with lenti-OLA1P2 using phosphorylated STAT3 (Tyr705) antibody. f RNA pull-down analysis to determine the interaction of phosphorylated STAT3 protein with a portion of OLA1P2 using 5′ biotin-linked RNAs. g Diagram showing the conserved STAT3 transcriptional response element in part 1 of OLA1P2. h RNA pull-down analysis to determine the interaction of phosphorylated STAT3 with mutated OLA1P2 in COLO205 cells. ***: P <0.001

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