Fig. 8

OND reduces access to chromatin by anti-histone antibodies and induces deacetylation of histones and a reduction in cellular granularity. HL-1 cells, either untreated or subjected to 1 hour of OND, were trypsinized to produce a monodisperse suspension, fixed, permeabilized, washed and immunostained with anti-histone H3 antibodies, as indicated. Cytometric analysis was performed on a minimum of 10⁴ cells. a A comparison of the staining intensity of untreated (UT) and OND cells; each data pair is normalized to the median of the untreated total H3 with the median and range of the distribution shown above each histogram. b Western blot analysis of total H3K14ac and of H3K14me3 across the experimental time-course. c The distribution of side-scatter measurements, which are proportional to internal cellular granularity, is presented for 10⁴ cells as box plots showing the median, 25 % and 75 % intervals as boxes and the 5 % and 95 % intervals as whiskers. The median value for untreated cells is shown as a horizontal line through all box plots. The z score between the untreated cell population and each other experimental condition, determined by the Mann-Whitney ranked sum test, is indicated by the color of the box according to the key on the right