Fig. 5
From: A transient ischemic environment induces reversible compaction of chromatin
OND induces chromatin compaction as determined by resistance to digestion by DNAseI. HL-1 cells, either untreated or subject to 1 hour of OND, were fixed, permeabilized and stained with 5 μM DRAQ5 for 30 minutes. Cells were then digested with 5 U/ml DNAseI at 37 °C with cellular fluorescence measured on a confocal microscope, with images generated every 4 minutes, observing 11 cells in total for each experimental condition, a.u. arbitrary units