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Fig. 5 | Genome Biology

Fig. 5

From: High-frequency, precise modification of the tomato genome

Fig. 5

PCR detection of one-sided and true GT events in plants derived from event 11. a Diagrams of true and one-sided GT events. Primers used for PCR are marked with numbered arrows. b PCR analysis confirmed one-sided GT events in plants 11.1, 11.2, 11.4 and 11.5 and a true GT event in plant 11.3. c Reconstruction of the one-sided GT event from plant 11.1. DNA sequence analysis revealed precise, HR-mediated repair on the right side. On the left side, before re-ligation of the broken chromosome, an additional 966 bp of sequence was copied from the GT vector and another 29 bp of unknown origin

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