Fig. 3From: TLE6 mutation causes the earliest known human embryonic lethalityThe TLE6 mutation diminishes SCMC protein binding and complex formation. a Interaction of endogenous OOEP protein with TLE6 from control and patients cells after immunoprecipitation with anti-OOEP and IgG antibody followed by immunoblotting. b Quantification of A from two biological repeats and two technical repeats. c Interaction of endogenous KDHC3L protein with TLE6 from control and patient cells after immunoprecipitation with anti-KDHC3L/Ecat1 and IgG antibody followed by immunoblotting. d Quantification of C from two independent experiments. e Immunoprecipitation performed from HEK293 cells expressing wild-type or mutant TLE6 S510Y with anti-Flag beads followed by immunoblotting, showing reduced binding of OOEP and KDHC3L to mutant TLE6. f Quantification of E from two independent repeatsBack to article page