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Fig. 3 | Genome Biology

Fig. 3

From: A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression

Fig. 3

Subcellular localization of FLAG-SNAP-TERT. IF analysis of fixed HeLa cells expressing FLAG-SNAP-TERT. The SNAP-tag was labeled with SNAP-Cell® 647-SiR dye (scale bar = 5 μm). Telomeres and Cajal bodies were stained with antibodies against TRF2 and coilin, respectively. Edited cells but not parental cells showed FLAG-SNAP-TERT foci that co-localized with telomeres and Cajal bodies. Two independent clones expressing FLAG-SNAP-TERT were used to generate the images shown

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Genome Biology

ISSN: 1474-760X

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