Table 2 HPLC analysis of tRNA^Val(AAC) nucleoside content from an S. cerevisiae trm82∆ strain expressing TRM82 variants

m⁷G

1

0.50 ± 0.07

0.53 ± 0.03

<0.03

0.45 ± 0.05

0.46 ± 0.07

Ψ

4

3.66 ± 0.10

3.87 ± 0.18

3.94 ± 0.18

3.89 ± 0.15

3.64 ± 0.08

I

1

0.76 ± 0.02

0.85 ± 0.07

0.91 ± 0.06

0.90 ± 0.05

0.77 ± 0.01

m⁵C

1

0.87 ± 0.03

0.94 ± 0.03

1.14 ± 0.05

0.97 ± 0.05

0.91 ± 0.03

m¹G

1

0.81 ± 0.05

0.98 ± 0.05

0.99 ± 0.05

1.00 ± 0.08

0.83 ± 0.05

m²G

0

0.03 ± 0.01

0.05 ± 0.01

0.54 ± 0.10

0.07 ± 0.02

0.05 ± 0.01

m⁷G

1

0.61 ± 0.04

0.50 ± 0.07

<0.03

0.25 ± 0.03

0.55 ± 0.11

Ψ

4

3.88 ± 0.10

3.93 ± 0.05

3.85 ± 0.15

3.99 ± 0.20

4.10 ± 0.08

I

1

0.80 ± 0.01

0.77 ± 0.02

0.74 ± 0.06

0.78 ± 0.07

0.85 ± 0.04

m⁵C

1

1.04 ± 0.04

1.19 ± 0.04

1.53 ± 0.08

1.42 ± 0.09

1.16 ± 0.07

m¹G

1

0.87 ± 0.01

0.79 ± 0.03

0.79 ± 0.10

0.87 ± 0.06

0.94 ± 0.01

m²G

0

0.05 ± 0.03

0.10 ± 0.03

0.45 ± 0.03

0.27 ± 0.05

0.06 ± 0.01