Table 1 HPLC analysis of tRNA^Phe nucleoside content from an S. cerevisiae trm82∆ strain expressing TRM82 variants

m⁷G

1

0.41 ± 0.04

0.34 ± 0.03

<0.03

0.21 ± 0.01

0.32 ± 0.04

Ψ

2

2.09 ± 0.06

1.94 ± 0.03

1.85 ± 0.04

1.99 ± 0.07

2.06 ± 0.05

Cm

1

0.89 ± 0.09

0.92 ± 0.05

0.93 ± 0.08

0.95 ± 0.03

0.93 ± 0.06

Gm

1

0.72 ± 0.01

0.76 ± 0.07

0.76 ± 0.03

0.73 ± 0.08

0.75 ± 0.02

m⁵C

2

1.53 ± 0.06

1.64 ± 0.07

1.69 ± 0.03

1.66 ± 0.06

1.59 ± 0.03

m²G

1

0.78 ± 0.02

0.83 ± 0.04

0.87 ± 0.02

0.83 ± 0.04

0.82 ± 0.03

m⁷G

1

0.37 ± 0.03

0.21 ± 0.03

<0.03

<0.03

0.18 ± 0.02

Ψ

2

2.00 ± 0.08

1.91 ± 0.06

1.87 ± 0.01

1.82 ± 0.10

1.92 ± 0.01

Cm

1

0.98 ± 0.08

1.00 ± 0.14

0.91 ± 0.02

0.93 ± 0.05

0.90 ± 0.04

Gm

1

0.77 ± 0.03

0.84 ± 0.02

0.91 ± 0.05

0.96 ± 0.02

0.84 ± 0.02

m⁵C

2

1.63 ± 0.03

1.69 ± 0.02

1.79 ± 0.05

1.76 ± 0.04

1.71 ± 0.02

m²G

1

0.82 ± 0.05

0.88 ± 0.02

0.90 ± 0.07

0.94 ± 0.06

0.86 ± 0.02