Fig. 5

Bcl11a-deficient HSCs show self-renewal defects. a Gene set enrichment analysis showing significant depletion of the self-renewal gene signature in Bcl11a ^−/− HSCs (p < 0.001). The normalized enrichment score (NES) of 1.64 indicates significantly higher self-renewal gene expression in the Bcl11a ^+/+ HSCs compared with Bcl11a ^−/− HSCs. b Percentage of donor cells in total nucleated peripheral blood (PBL) cells along with time after transfer. We injected 2000 Bcl11a ^+/− or Bcl11a ^−/− LSKs (CD45.1⁻) with 1.0 × 10⁶ bone marrow (BM) cells (CD45.1⁺) into sublethally irradiated recipient mice (CD45.1⁺). c Comparison of the number of donor LSK cells and HSCs in secondary recipient mice 18 weeks post-secondary transfer; *p < 0.05, **p < 0.01. d FACS dot plot of donor Bcl11a ^+/− and Bcl11a ^−/− HSCs (LSK CD150⁺48⁻ and LSK CD34⁻135(Flt3)⁻) in secondary recipient mice. BM cells were analyzed 18 weeks post-secondary transfer. Bcl11a ^+/−, CreERT2; Bcl11a ^+/flox (treated with tamoxifen); Bcl11a ^−/−, CreERT2; Bcl11a ^flox/flox (treated with tamoxifen). In panels (b) and (c), at least three mice were used for each time point or each cell type in independent experiments. The error bar represented mean ± 1 standard deviation