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Table 1 Evaluation of total RNA and circRNA absolute quantity by ERCC spike-in RNAs

From: Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos

Stage Oocyte Zygote 2-cell 4-cell 8-cell Morula Blastocyst
Average number of mRNA molecules per embryo (×106) 26.8 21.3 4.5 5.7 6.6 13.8 21.1
Average number of circRNA molecules per embryo 2278 1850 1509 1422 1602 2799 779
Ratio of circRNA/host linear RNAR 1.6 % 1.7 % 2.2 % 4.7 % 4.2 % 6.8 % 9.8 %
Average number per type of circRNA transcript per embryo 71 64 24 15 21 41 158
Number of circRNA genesa per stage 363 425 684 517 433 474 123
Number of types of circRNA transcripts per stage 591 718 1,243 861 702 721 140
  1. The total mRNA copy number and circRNA copy number were evaluated through ERCC spike-in
  2. aCircRNA host genes; some host genes may generate several different types of circRNA transcripts