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Figure 3 | Genome Biology

Figure 3

From: The histone methyltransferase SDG8 mediates the epigenetic modification of light and carbon responsive genes in plants

Figure 3

SDG8-binding of hypomethylated genes. (A) HA-tagged wild-type SDG8 was transformed into sdg8-5 mutant background to allow detection of direct binding of SDG8 to the H3K36 hypomethylated genes by anti-HA ChIP-Seq. Examples of anti-HA ChIP-Seq profiles on H3K36 hypomethylated genes are shown, supporting that SDG8 directly binds to its functional targets. All genes are visualized with 5′ on the left, and 2 kb flanking intergenic regions or until the neighboring gene are also shown. (B) Intersection of 2,267 SDG8-bound genes and the 4,060 hypo-methylated genes in sdg8-5 versus WT identified 728 SDG8-bound and hypomethylated targets (a.k.a. ‘SDG8 direct targets’). (C) Significantly over-represented cis-regulatory motifs G-box (bZIP binding motif) and FORC A are identified in the promoters of the 728 direct targets of SDG8 (bound by SDG8 and hypomethylated in sdg8-5).

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