Figure 3

Regulation of mRNA/protein expression decoupling by RBPs. (A-D) Enrichment of RBP binding sites within discordant genes in each of the four main expression patterns, compared with concordant genes from the same pattern. The x-axis shows the enrichment fold change based on the binding site number; the y-axis shows the significance of the binding site density difference. Each circle represents one RBP. The circle radius shows the proportion of discordant genes targeted by the RBP within the group. The colors indicate RBPs showing significant enrichment (red), no difference (grey) or significant depletion (blue) of binding sites within discordant genes compared with concordant ones. See also Figure S9 in Additional file 1. (E-H) The average mRNA (green) and protein (blue) expression, as well as expression of RBP genes showing significant enrichment of binding sites among discordant genes (red) in each of the four expression patterns. The curves are calculated using cubic spline regression. The symbols show the mean expression in each individual. The y-axis shows mRNA and protein expression, normalized to the mean and standard deviation of corresponding expression levels in the developmental interval. The RBPs and the numbers of target genes in each group are shown on the top of the panels. The vertical dashed line marks separation of the developmental and aging intervals. See also Figure S10 in Additional file 1.