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Figure 1 | Genome Biology

Figure 1

From: Bridger: a new framework for de novo transcriptome assembly using RNA-seq data

Figure 1

Flowchart of Bridger. (a) The algorithm takes RNA-seq reads (single or paired) to assemble splicing graphs, each of which provides a complete representation of all alternative splicing transcripts for each locus. (b-d) Each splicing graph is processed independently. (b) Each edge in a splicing graph represents one splice junction. In this example, edges 1 and 3 are compatible, while edges 3 and 4 are not compatible. (c) A compatibility graph. (d) A minimum path cover model is applied to recover a minimal set of transcripts that could be tiled together through overlapping sequence reads and ‘explain’ all observed junctions in a splicing graph.

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