Figure 5

Cellular localization of divergent lincRNAs and their neighbors. (a) Two-color overlay micrograph presenting florescence probes targeting the lincRNA (green) and coding neighbor (red). Co-localized spots are marked yellow. The lincRNA and cell type are marked on the image. Scale bar, 5 μm; marked on the left most image. Top: illustration of the positional genomic orientation of a divergent lincRNA and its coding gene neighbor. (b) Representative fluorescence micrographs as shown in Figure 2 for the lincRNAs in a. Scale bar, 5 μm. (c) Scatter plots of the relationship in each cell between the expression level of the lincRNA (X axis, molecule count) and that of its neighboring coding gene (Y axis). Pearson correlation coefficients (r) after removal of outliers (Additional file 1) are denoted on top. Data in (a-c) are presented for eight of the nine lincRNA-gene neighbor pairs for which a valid probe set exists.