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Figure 6 | Genome Biology

Figure 6

From: Chromosome 7 gain and DNA hypermethylation at the HOXA10 locus are associated with expression of a stem cell related HOX-signature in glioblastoma

Figure 6

Epigenetic features of the HOXA10 and HOXA9 promoters. ChIP-qPCR is shown for three different histone marks, two associated with active transcription/open chromatin (H3K4me3, H3K36me3) and one indicative of transcriptional repression (H3K27me3), in our four glioma sphere lines at the HOXA10 promoter (A) and the HOXA9 promoter (B). The measurements represent relative enrichment over IgG control (error bars represent SEM in duplicate experiments). Methylation-specific clone sequencing is shown for the CpGi located in the promoter of HOXA10 in three primary GBM (C) and respective derived glioma sphere lines (D). The HOXA10 promoter of HOX-high GBM-2207 and GBM-2669, and the corresponding GS lines (LN-2207GS, LN-2669-GS) is unmethylated, in contrast to the HOX-low GBM-2540 and its corresponding GS line which exhibit both a highly methylated HOXA10 promoter. (E) The CpGi located in the promoter of HOXA9 is highly methylated in all three glioma spheres lines. Gray boxes represent methylated CpGs.

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