Figure 2
From: Quantification of cell identity from single-cell gene expression profiles
Information-based index of cell identity robustly assigns cell type to single cells. (A) Spec score with increasing marker set sizes in different root tissues measuring level of transcript uniqueness in each cell type. CC, Companion Cell; LRM, Lateral Root Meristem; PPP, Phloem-Pole Pericycle; XPP, Xylem-Pole Pericycle. (B) ICI variability as a function of the number of markers used. Variability is measured as the Euclidean distance between ICI vectors for cumulative information threshold i and i + 1. (C) ICI signal for the 30 plant cells used in the study as a function of the number of markers. (D) ICI output for the single cells used in this study, showing the predicted identity in the heatmap and the cell type marked by the reporter used to collect the cells. (E) Expression of selected markers for atrichoblast, stele, and QC in the single cells. Note the overall tendency of marker specificity and substantial noise from either technical or biological sources. (F) Proportion of cell identities called correct (red), unidentifiable (black), or incorrect (blue) at varying sequencing depths.