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Figure 1 | Genome Biology

Figure 1

From: Modulation of microRNA editing, expression and processing by ADAR2 deaminase in glioblastoma

Figure 1

Down-regulation of selected onco-miRNAs (miR-221, miR-222 and miR-21) in glioblastoma cell lines upon ADAR2 expression. (a) Mature miR-221, miR-222 and miR-21 expression levels are shown in untreated (ctrl, dark gray), ADAR2 over-expressed (ADAR2, medium gray) and inactive ADAR2 over-expressed (ADAR2 E/A, light gray) U118 cell lines. Values represent the mean of at least three independent quantitative RT-PCRs (qRT-PCRs). Error bars represent standard error of the mean (s.e.m.) (n = 3), **P < 0.01, *P < 0.05. (b) The same experiments shown in (a) were performed in the A172 cell lines. (c) Mature miR-223 expression levels are shown in U118 (upper panel) and in A172 (lower panel) cell lines. Mean ± s.e.m. (n = 2). All the samples were normalized to RNU6B levels. The expression levels were calculated as a relative-fold increase compared with untreated cells (ctrl) arbitrarily set to 1.

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