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Figure 3 | Genome Biology

Figure 3

From: CIRI: an efficient and unbiased algorithm for de novo circular RNA identification

Figure 3

A non-exonic circRNA with intronic/intergenic circRNA fragments. (A) Coverage of one exon and three intronic regions contained in chr10: 60,347,975|60,380,661 in the two samples (red, junction reads identified by CIRI in RNaseR-; blue, junction reads identified by CIRI in RNaseR+; grey, other reads). (B) circRNA structure and its two linear amplified fragments using a pair of outward-facing primers. The top PCR product in the gel is longer than one complete circle around the circRNA and the bottom PCR product is shorter than one complete circle. (C) Sequencing chromatogram of the PCR product across the junction. (D) The circRNA contains three intronic circRNA fragments and one exon, which are all flanked by GT-AG splicing signals. The positions of stop codons in all six frames are shown as crosses.

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