Figure 2

Heat shock response in N. coriiceps . (A) Tissue specific expression of HSR-related genes in various tissues. Multi-chaperone complexes of HSP90 (FKBP and PTGES), SIRT1 (NAD-dependent decetylase sirtuin-1; SIRT1 is negatively related to the acetylation of HSF1 in humans), SUMO, HSP90-alpha, and UBC9 were expressed in blood and other tissues. The SUMO-cconjugation enzyme ubiquitin carrier 9 (UBC9) discriminates between the phosphorylated and non-phosphorylated PDSM of HSF1. (B) HSF1 and its post-translational modification sites in N. coriiceps. Serine corresponding to ser303 of human HSF1 was substituted with asparagine. This site is responsible for sumoylation of lys298 of PDSM in human hsf1. In Xenopus sp. and G. aculeatus, other amino acids were identified at the site corresponding to ser303 of human HSF1. All sequenced mammals and most fish have serine at this site. This substitution of serine to asparagine was also identified in icefish and Dragon fish in the Antarctic Ocean. (C) Components responsible for the HSR in N. coriiceps and its regulation.