Figure 2

Methods for isolating single cancer cells from abundant and rare populations. (a) Methods for isolating single cells from abundant cellular populations include: micromanipulation by robotics or mouth pipetting, serial dilutions, flow-sorting, microfluidics platforms and laser-capture microdissection (LCM; 63X objective). (b) Methods for isolating single cells from rare cellular populations include: CellSearch (Johnson & Johnson), DEP-Array (Silicon Biosciences), CellCelector (Automated Lab Solutions), MagSweeper (Illumina) and nano-fabricated filters (Creatv MicroTech).